7.2 RNA Sequencing

RNA sequencing is the process of identifying RNA expression in a given tissue of an organism. An experiment utilizing RNA sequencing normally involves mutliple specimen and multiple tissues. A more elaborate approach would be to also include RNA expression over time, which would of course require the specimen to be alive and would for obvious reasons be much more difficult and expensive. Regardless, RNAseq can give valuable insights into the changes in RNA expression levels that might indicate disease or behavioral change. To learn more see Illumina’s website or RNA-Seq on Wikipedia.

For our purposes, RNA sequencing is normally carried out by the Genomics Core. So the only required molecular work is RNA isolation.