6.3 DNA Sequencing

We generally outsource DNA sequencing in our lab to the Genomics Core for whole genomes, or through third party services for sequencing single genes via Sanger sequencing. Visual analysis can be done for homologous strands of DNA by first aligning the sequences of interest, and then using special software to look at each base pair in order to identify SNPs or other mutations. Using this type of observation method is usually preserved to single gene sequences. For analysis of whole chromosomes or genomes, we usually use a more advanced bioinformatics approach in order to obtain more holistic and objective results.